All About Sample Submission for DNA Services

New MRCF User

If you have never submitted samples to the MRCF before, there is a one-time form that you are required to fill-out. On this form, you and your PI or Lab Director will provide contact information about your lab and samples information to ensure our staff's safety while handling your samples.

NEW USER FORM

Job Request Form

 The Job Request Form is a form that contains information about the samples you are sending us - i.e. Sample names and what kind of samples we are processing. For more information on how to prepare your samples, checkout the section below "Sample Concentration and Primers."

 MRCF Job Request Form

NGS Job Request Form

We provide the following primers for Sanger DNA sequencing, Next-Generation Sequencing and typical Polymerase Chain Reaction (PCR). 

Be sure to use 1uL of a 3.2 uM primer per 5uL of sample in a Sanger reaction. If we are performing a DNA clean-up or need to quantify the sample, do not add primer directly to the sample (please provide primer in a separate tube).

If you would like us to add these to your samples, it must be specified on the Job Request Form.

Tubes:

Samples can arrive in strip-tubes, microcentrifuge tubes or other properly sealed tube. We ask that the tubes be wrapped in Paraffin to prevent any lids opening and evaporation.

Small tubes, such as PCR tubes, have arrived crushed in the mail. If you are sending small tubes, please secure them in something larger - either place them in a 50mL conical tube, or some sort of rack, to prevent damage from the shipping and handling.

Labeling:

Clearly label your samples. If samples are in strip-tubes, mark the correct orientation by placing an "A1" or "1" to show which is the first sample in the strip. If samples are in a plate, make sure the plate is marked so we know typical A1-H12 orientation was followed.

If sending primers, makes sure the primer concentration is clear. We can dilute primers to the correct concentration if we know what the concentration is for the primer sent to our lab.

Temperature:

DNA for Sanger sequencing and Fragment Analysis can be shipped at room temperature (ambient) if the samples will arrive within a day or two. If delays are expected, please pack with ice packs to prevent the samples from getting too hot. DNA libraries for NGS should be sent on ice to prevent adapter degradation.

RNA needs to be kept frozen. Overnight shipping on dry ice is preferred, however, if you are certain your samples are not contaminated with RNAses, shipping on ice is an option.

Sample Ship to address:

Idaho State University
Molecular Research Core Facility
Gale Life Sciences Building
640 Memorial Drive Mail Stop 8007
Pocatello, ID 83209-8007

Please email an electronic copy of your Job Request Form to mrcf@isu.edu and include a hard copy of the form along with the shipped samples.

Downloading Data

Once your data has been generated, the raw data files will be uploaded to BOX. You do not need to have a BOX account to access your data. We will email a link to the folder containing your files once the data has been finalized.

Viewing Data

There are freeware software packages that can be downloaded for data viewing:

• Sequence Scanner for Sanger Sequencing data files
• Peak Scanner for Fragment Analysis data files

The MRCF also has an in-house computer with software installed to analyze sequence and fragment analysis data: Sequencher, for sequencing data and GeneMapper v5.0, for fragment analysis data. Analyzing your data sets on our in-house computer is free of charge.

Data Retention Policy

Data will only be saved for 1 year and will not be archived. Please back-up your data immediately.