Idaho Falls Stockroom

CHE 107/108

Mondays - Thursdays
September - April, 8 am to 4 pm
David Barber, Laboratory Supplies Supervisor
barbdavi@isu.edu
282-7886

Policies

A

Access:

  1. The main stockroom is in the rear of CHE 108.  Lab instructors teaching in CHE 107 should not help themselves to anything in CHE 108 without talking to the lab materials supervisor.
  2. If you teach a lab in CHE 108, your key to the CHE 108 door will also work on the CHE 108 stockroom.
  3. There is a small stockroom in the rear of CHE 107, which is always accessible by the lab instructors for labs held in that room.  If it is locked, your key to the CHE 107 door also works on that door.  Extra supplies for your labs may be available in that small stockroom.
  4. Turn off the lights and lock the doors to CHE 107 and/or 108 when your labs are finished. You must use a key to lock the door (NOT the push button lock).
  5. The other exit door in the rear of CHE 108 leads to the chemistry lab.  This door is for emergencies only where evacuation through the main lab door is not possible.  Use of this door sets off the motion detector in the chemistry lab and alerts public safety.
B

Teaching Needs:

  1. Supplies requested from the lab materials supervisor will be provided on a cart before the first lab of the week.  It is the responsibility of the first lab instructor of each lab to set out the materials for the lab in a way most conducive for student use during the lab.
  2. If an item appears to be missing from the cart:
    • Check the refrigerators in the front of the labs for chemicals that may need refrigeration.
    • Check the student benches, back island or wall benches, for larger items that may not fit on the cart
    • Check the shelves under the island in CHE 107 or the wall shelves in CHE 108 for extra disposable supplies such as Kim wipes, disposable pipettes, etc.
    • Check other storage areas:  gloves are found in plastic cases mounted to the walls of the labs; goggles are found on the shelf near the door (CHE 108) or on the shelf under the back bench (CHE 107); paper towels, Kim wipes, handsoap, DI water bottles, and lab bench cleaner are next to every sink
    • Microscopes are always in the microscope cabinet; the key to the cabinet is in the drawer at the front desk of each lab.  Note that to open a cabinet door without a lock, you must reach in through the door you unlocked and release the latch for the other door from the inside.  Microscope cabinet doors do not require brute force to open!
  3. For all unusual occurrences (e.g. spills and shortages of stock) that occur when the lab materials supervisor is not available to assist, lab instructors are asked to use good judgement in attempting to resolve their challenge (e.g. have students work in larger groups) and leave a note on the front bench for the lab materials supervisor.
  4. No lab supplies of any kind should be removed from the labs, except for field labs supervised by the instructor.  All wastes are disposed of in the lab where they are generated.
C

Research or Outreach Needs:

  1. Supplies for projects not associated with a class generally need to be purchased using outreach- or research-allocated funds.  For a small experiment requiring small amounts of a chemical, or for outreach activities that require small quantities of disposable items such as gloves or paper towels, exceptions to this rule may be made at the discretion of the lab materials supervisor.
  2. Students conducting independent projects may use lab equipment, but it generally must be used in the CHE 107 or CHE 108 labs and may not be removed from campus.
  3. Simple field or greenhouse equipment may be checked out to students only with permission.  Student grades will not be released until equipment is returned.
  4. Specimens and equipment borrowed for outreach activities must also be checked out from the lab materials supervisor or a faculty member.
D

Labelling:

  1. All chemicals must be labelled.  Any bottle, flask, beaker, test tube, or another container with liquid or solid chemicals (including water!) must be labelled at all times.
  2. If you prepare a stock chemical yourself, the bottle must include the following:
    • Chemical name
    • Concentration of chemical (and diluent if other than water)
    • Date of chemical preparation
    • Initials of the person who prepared the solution
  3. For students using chemicals in the lab, they must label all lab containers they use (beaker, test tube, etc.) in a way that leaves the contents unambiguous (given chemicals available for that lab) and in a way that is easily removable at the end of lab—using a wax pencil or a piece of tape.
  4. Students are responsible for removing labels after lab from all glassware that they use.  Wax pencil marks must be rubbed off using a dry paper towel.  The tape should be removed and discarded.  Note that wax pencil marks are more easily removed if they are not written on the white area of the glassware.  No indelible markings should be used.
E

Chemical Solutions: 

  1. Please contact the stockroom if solutions are getting low and there will not be enough for the following labs that week.
  2. If any solutions are not working properly, let the stockroom know and a new solution will be prepared or ordered as quickly as possible.
 
F

Equipment Problems:

  1. If a microscope is not working properly, let the stockroom know the microscope # so it can be cleaned.  If it is still not working, we will arrange for its repair. 
  2. If another piece of equipment is not working properly, let the stockroom know or leave a note.  Indicate whether it is a calibration problem or some other type of problem so the proper steps can be taken to fix it.
  3. If you do not know how to use a piece of equipment, ask for help from the lab materials supervisor (David) or a faculty member.
G

Waste Chemical Disposal:

  1. Absolutely no chemicals other than soap and water may be dumped into sinks in Idaho Falls.  However, once the bulk of a chemical has been dumped into a waste container, the remaining residue in glassware may be washed/rinsed and the wastewater may go down the sink. 
  2. All waste must be left in the room in which it is generated.
  3. Waste bottles for all expected wastes should be requested by the faculty member in charge of the lab along with other lab supplies.
  4. Labelled waste bottles will normally be found under the hood of each lab.  If excess waste is generated and the lab materials supervisor is not available, use any available container and label it appropriately (see other waste containers for examples).
  5. All waste containers are left in the hood at the end of all labs.  Instructors should not empty or move waste containers.
H

Sharps and Uncontaminated Broken Glass: 

  1. Discard only used metal items (needles, scalpels, razor blades) in the sharps container. 
  2. Discard only glass free of biological contamination in the broken glass container.  Glass with only small chips can be sanded down for safety and continue to be used.  Leave chipped glass in the back prep room with a note.
  3. Lab instructors do not need to empty or move sharps or broken glass containers.
A

Biologicals: 

  1. Bacteria, blood, and animal tissue will have biohazard collection containers available in the hood, which must be requested by the faculty member when submitting supply requests.  Leave the biohazard container in the hood at the end of lab.
  2. Leave lids loose on living cultures, unless transporting them to Pocatello (tighten lids for the journey and then loosen them again when you arrive).  Only transport organisms if you have specifically been requested to do so.  If organisms are to remain in Idaho Falls, leave a note stating whether they should be kept or disposed of.
  3. Treat all living and dead animals with respect.  Callously hacking into organisms, arranging them into “humorous” poses, or making jokes about living or dead organisms is unacceptable behavior.
B

Preserved Animals:

  1. A container for a collection of preserved animal tissue should be requested by the faculty member in charge of the lab.  This container will be located in the hood.
  2. After all of the week’s labs are complete (or when full, or when it starts to smell), tie the bag tightly shut, label it, and put it in the freezer. 
  3. Leave a note for the lab materials supervisor when the freezer is full or no more animal waste will be generated during the semester, so that pickup may be arranged.
C

Dirty Slides:

  1. A “dirty slide” container is provided in the back of the lab for disposal of student-created non-hazardous wet mount slides.  Plastic coverslips can be disposed of in the trash.
  2. Request a biohazards container for slides that require it.
  3. Please ensure that commercially prepared slides do not go in these containers.
  4. Lab instructors do not need to empty or move dirty slide containers.
D Accidents: All accidents should be reported using the accident form and filed with the Laboratory Materials Supervisor on your campus and the lab coordinator.
E

Cleanup Expected of Students:

  1. Remove all markings from empty glassware (wipe off wax pencil marks with a dry paper towel; remove all tape).  Clean/rinse glassware so it is ready for the next lab.
  2. Dispose of only appropriate items in the trash (no chemicals, biohazards, sharps, glass):  gloves, paper towels, Kim wipes, lens paper, plastic coverslips, scraps of parafilm, and other paper/plastic trash are ok.  Our labs do not contain anything so hazardous that small amounts of chemical contamination on gloves or paper towels are hazardous.  If a paper towel is completely saturated with a chemical, you may leave it with the chemical waste in the hood.
  3. Return any prepared slides to the proper boxes.
  4. Return microscopes in the proper condition to correct cabinet (# on microscope must match # on cabinet).
    • Cord is wrapped neatly and tucked in
    • Lowest power lens is in place
    • Empty stage (no slides)
    • Aligned arm & eyepieces (head not rotated)
    • Racked down & centered stage
    • Then make sure students add the dust cover and return them to the proper cabinet number.
  5. Log off the computer and shut the lid.  These computers are on the ISU system and if a student fails to log off, they will be blocked from logging on to any other computer on campus.
  6. Other materials
    1. If there are more lab sections during the week:  students should leave all materials where they found them when they arrived in the lab.  If it was on their bench, they should leave it there.  If it was on the back bench, it should be returned there.
    2. For the last lab section of the week:  Return all materials to the large island in the back of the lab.  Only the following items should be left on the student benches next to the sink:  DI bottle, alco Nox wash bottle, handsoap, and Kim wipes
  7. Wipe down lab table using alco Nox and paper towels.
  8. Push in lab stools.
F

Cleanup Expected of Lab Instructors:

  1. Ensure that students are putting their waste in the appropriately labeled containers in the hood.  Absolutely no chemicals should be left in general lab glassware at the end of a lab.  Only stock chemical bottles and labeled waste containers should contain chemicals after a lab.
  2. Ensure that students have removed all markings from glassware and cleaned/rinsed them so they are suitable for the next lab section.
  3. Check prepared slide boxes to make sure that all prepared slides are accounted for.  If a prepared slide has been broken during a lab, leave a note (and then put it in the broken glass).  Small chips can be sanded down to make them safe and the slides can still be used.  Leave a note if a slide has a chip and needs to be sanded for safety.
  4. Check that the microscopes have been put away properly (in the proper condition and in the proper cabinet) and lock the cabinets.
  5. If there are more lab sections after yours, make sure that all glassware, chemicals, and equipment have been returned to the proper place in the proper condition (clean!) for the next lab. Notify the lab materials supervisor if chemicals or supplies are running low.
  6. If you have the last lab of the day (even if it is not the last lab of the week), return all perishable items to the refrigerator and turn off all electrical equipment (water baths, electrophoresis equipment, spectrophotometers).
  7. If you have the last lab of the week, put all glassware, chemicals, and equipment back on the supply cart.  All items should be clean, but glassware does not need to be spotless, as it will go through the dishwasher prior to reuse.  The cart can be left in place in the lab.  It does not need to be moved elsewhere.  Large items (spectrophotometers, balances, electrophoresis equipment, laptops) may be left on the large back island.  The only things that should be left on student benches at the end of the week’s labs are these items that are always next to the sinks: DI water bottle, alco Nox wash bottle, handsoap, and Kim wipes.
  8. Turn off the projector, log off the instructor computer, and close the computer.
  9. Turn off the lights and lock the doors to CHE 107 and/or 108 when your labs are finished.  Note that the push-button lock on the inside of the door only locks the door when it is already closed (this allows students to lock the door from the inside if there is an active shooter event).  If you push the button lock while the door is open, it unlocks when the door closes.  Thus, if you are on the outside of the door, you must use a key to lock the door (NOT the push button lock).
G

End of Semester:

  1. Clean out drawers in front bench.
  2. Ask lab materials supervisor if any other cleanup needs to be done.
A

Contaminates:

  1. Contaminated glassware:  There is a waste collection area in the back of the lab on the bench next to the hood for glassware contaminated with biological waste.  Place the following items in this waste collection area:
    • Test tubes with broth or slants (extra racks are on the shelves near the lab) but have students remove all tape labels and wax pencil marks first.
    • Flasks – keep the aluminum foil on each flask
    • Broken test tubes or other broken glass with biological contaminates–place inside an autoclave-safe container
  2. Contaminated slides – dirty slide box on back bench
  3. Contaminated plastic or other disposable items:  place the following items directly in the red biohazard bins.  If the bin is getting full, notify the lab materials supervisor (David).
    • Microbiology plates
    • Oxidase slides
    • Pipette tips, cotton swabs, gloves, paper towels
B

Dye spills:  Clean up spills promptly with Eradosol found in the trays with the microbiology stains.

C

Accidents: All accidents should be reported using the accident form and filed with the Laboratory Materials Supervisor on your campus and the lab coordinator.

D

Glass Pipettes:  Used glass pipettes should be submerged in the white plastic pipette collection containers found in the lab.  These can be left in place at the end of the lab/week.

E

Autoclaves:   

  1. All supplies for student labs that require autoclaving will be provided by the lab materials supervisor (David).  Generally, extras are provided.
  2. If a student has a project requiring the use of the autoclave, schedule a time with the lab materials supervisor to use the autoclave and obtain training on its use.
F

End of Lab:

  1. Return all unused media to the “clean” refrigerator.  Blood agar plates especially need to be refrigerated. 
  2. Cultures to be kept should go in the “dirty” refrigerator.
  3. Instruct students to dispose of all contaminated items no longer needed at the end of the lab – do not leave biohazard items on the lab benches.
  4. Prepared slides must be returned to the proper box.
  5. Microscopes must be put away in proper condition (knobs, lenses, stage, cord in proper position; no slides left on them!) in the proper cabinet (# on microscope matches # of cabinet).
  6. Make sure all gas is turned off.
  7. If there are more labs that week, return all other items to where students found them, clean and ready to be used again.
  8. If you have the last lab of the day (even if it is not the last lab of the week), return all perishable items to the refrigerator and turn off all electrical equipment (water baths, electrophoresis equipment, spectrophotometers).
  9. Return all dye bottles to their tray.
  10. Clean all bench tops with CiDecon
  11. Push in lab stools.
G

End of Week:

  1. If it is the last lab of the week, return all items to the cart near the front bench. The cart can be left at the front of the room.  Items should be left clean, but glassware will be washed in the dishwasher prior to reuse.  Larger items (balances, microfuges, water baths, etc.) can be left on the counters on the side or back wall. 
  2. Only the following items should be left on the student benches next to the sink:  DI bottle, disinfectant wash bottle, handsoap, kimwipes, lens paper, and Bunsen burners
H

End of Semester:

  1. Empty the refrigerators and incubators of bacterial cultures.
  2. Make sure that all bench tops are clean (this means no biohazard present, no old tape, no used Kimwipes, etc.)
  3. Clean out drawers in front bench.
  4. Ask lab materials supervisor if other cleaning needs to be done.

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