EDUCATION

1991-1996 Medical College of Wisconsin, Department of Biochemistry, Milwaukee, WI

• Ph.D. in Biochemistry, January 1997
• Research Advisor: Henry M. Miziorko, Professor, Ph.D.

1986-1991 University of Wisconsin-Stevens Point, Departments of Chemistry and Biology, Stevens Point, WI

• B.S. Chemistry (ACS) and Biology, May 1991

PROFESSIONAL EXPERIENCE:

2006-present ASSOCIATE PROFESSOR, Chemistry Department, Boise State University, Boise, ID
2000-2006 ASSISTANT PROFESSOR, Chemistry Department, Boise State University, Boise, ID

• Teaching: General Chemistry, Biochemistry, Seminar, Enzyme Kinetics and Mechanism, Bioinformatics.
• Research: Two projects involving the study of alcohol dehydrogenase, carbonyl reductase
• Performed research with 48 undergraduate students
• Advisor to 1 interdisciplinary masters student: Christopher Mark Maupin, title of Masters thesis, "Contribution of Position 228 in Horse Liver Alcohol Dehydrogenase to Coenzyme Binding". His degree was completed in 2002.
• Served on the graduate thesis committee for Mark Headley, a Biology Masters student, finished in 2005.
• Participated in teaching or taught solely the following graduate courses: CHEM 597 – Special Topics – Enzyme Kinetics and Mechanism, CHEM 431G Biochemistry I, CHEM 433G Biochemistry II, BIOL 546 Bioinformatics.
• Granted Tenure in 2006 and promoted to Associated Professor, effective July 1, 2006.

1997-2000 POSTDOCTORAL FELLOW, Department of Biochemistry, The University of Iowa, Iowa City, IA

• Applied steady state and transient enzyme kinetics, and site-directed mutagenesis techniques to study cooperativity in alcohol dehydrogenase
• Expressed and purified recombinant protein from both yeast and bacteria
• Optimized conditions for protein crystal growth
• Used a stopped-flow spectrophotometer and a fluorometer
• Utilized software to model protein structure (O), fit data (Fortran programs of Cleland and Nonlin), conduct bioinformatic searches (GCG) and simulate chemical reactions (Kinsim)
• Mentored an undergraduate students during their summer research fellowships

1991-1996 GRADUATE STUDENT/RESEARCH ASSISTANT, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI

• Applied steady state kinetics, active site directed affinity labeling, inhibitor design, and protein chemistry to study the enzymatic mechanisms of phosphoribulokinase and 3-hydroxy-3-methylglutaryl-CoA synthase
• Prepared coenzyme A thioesters, thioethers, sulfoxides, and analogs
• Used HPLC, FPLC, anaerobic chamber, and NMR
• Trained lab personnel

1991 SEMESTER BREAK RESEARCH PROGRAM PARTICIPANT, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI

• Applied steady state enzyme kinetics to study the conjugation of ubiquitin to target proteins

1990 DOE SCIENCE AND ENGINEERING RESEARCH SEMESTER FELLOW: Argonne National Laboratory, Chemistry Building, Argonne, IL

• Synthesized organic superconductors using organic and inorganic synthetic procedures that involved electrocrystallization

1987.1988 UNDERGRADUATE TEACHING ASSISTANT: University of Wisconsin- Stevens Point, Biology Department, Stevens Point, WI

• Taught transmission electron microscopy, sample preparation, and photography
• Conducted research project on geotropism in plants

1987.1988 TUTOR: University of Wisconsin-Stevens Point, Biology Department, Stevens Point, WI

• Tutored botany to undergraduate students

AFFILIATIONS:

2000-present American Chemical Society
2000-present AFFLIATE MEMBER, Mountain States Medical Research Institute
2000-present Idaho Academy of Science

AWARDS AND HONORS:

1986 Phi Eta Kappa Freshman Honor Society
1990 Department of Energy, Science and Engineering Research Semester Fellowship, Argonne, IL
1991 Brian Eagon Research Award, Biology Department, University of Wisconsin-Stevens Point
1993-1994 American Heart Association Predoctoral Fellowship
1995-1996 American Heart Association Predoctoral Fellowship
1997-1998 U.S. Department of Health and Human Services, National Heart, Lung, and Blood Institute Postdoctoral Training Fellowship
1998.2000 U.S. Department of Health and Human Services, National Institute on Alcohol and Alcohol Abuse Individual Postdoctoral Training Fellowship

GRANTS

"Biophysical and Biochemical of Protein Structure and Interactions." Project Team Leader, $150,000 period 7/01/05-6/30/07. Office of Research Administration, Boise State University.

"Anthracycline Specificities of Carbonyl Reductases" Principal Investigator, $100,000 Period: 7/01/2003 6/30/06 Funding Agency: NIH/NCI 1 R15 CA102119-01
The goal of this grant is to identify, purify and kinetically characterize the enzymes with anthracycline reductase activities that are present in rabbit heart.

"Structure/function analysis of anthracycline reduction by human carbonyl reductase." Magnet Principle Investigator My portion is for about $376,000 Period 7/01/04-6/30/09. This is a subproject that is part of the Idaho BRIN renewal entitled Idaho-INBRE, NIH/NCRR P20-RR16454

"Synthesis of doxorubicin C14 benzyl ethers and evaluation as carbonyl reductase substrates and topoisomerase II inhibitors." Co-Principle Investigator, $5000 Period 5/04 – 5/05 Mountain States Tumor and Medical Research Institute.

"Anthracycline Reduction by Human Liver Carbonyl Reductase: Determination of the kinetic mechanisms and substrate/inhibitor specificities" Principal Investigator, $70,000 Period: 9/01/2003-8/31/04 Funding Agency: Subproject of NIH-BRIN Grant # P20RR16454
The goal of this grant is to quantitatively determine the kinetic mechanisms of anthracycline reduction by human carbonyl reductase. Structure/function studies will be used to understand what accounts for the inhibitor and substrate specificities of the enzyme. Another goal of this grant is to generate significant preliminary data for submission of an R01 proposal to the NIH.

"Mechanisms of Anthracycline Pharmacokinetics and Aging" Co-Principle Investigator, $557,400 Period: 10/1/2003-9/30/07 Funding Agency: VA Merit Review Grant
The major goals of this project are to study the effects of aging on the activity of enzymes involved in anthracycline metabolism and transport.

"Mechanistic Studies of the Peroxisomal Multifunctional Proteins I and II" Principal Investigator, $38,840 Period: 7/01/2001-6/30/2004 Funding Agency: Research Corporation Cottrell College Science Award No. CC5404.
The goal of this grant is to understand the kinetic mechanisms of multifunctional proteins I and II, in order to gain insight into their roles in bifunctional protein deficiency.

"Inhibitor Specificities of Human Liver Carbonyl Reductase" Principal Investigator, $5000 Period: 5/12/2003-5/11/2004 Funding Agency: Mountain States Tumor and Medical Research Institute
The goal of this grant is to design and test human liver carbonyl reductase inhibitor candidates.

"Anthracycline Reductases from Rabbit Heart" Principal Investigator, $5000 Period: 7/01/2002-12/31/2003 Funding Agency: Boise State University Faculty Research Grant
The goal of this grant is to identify, purify and kinetically characterize the enzymes with anthracylcline reductase activities that are present in rabbit heart. Continue the work from the grant listed below.

"Purification and Characterization of Anthracycline Reductases from Rabbit Heart." Principal Investigator, $5000 Period: 7/01/2001-6/30/2002 Funding Agency: Moutain States Tumor Institute/Mountain States Medical Research Institute
The goal of this grant is to identify, purify and kinetically characterize the enzymes with anthracylcline reductase activities that are present in rabbit heart.

"Purification and Characterization of Recombinant Human Liver Carbonyl Reductase" Principal Investigator, $5000 Period: 7/01/2002-6/30/2003 Funding Agency: Mountain States Tumor Institute/Mountain States Medical Research Institute
The goal of this grant is to develop a recombinant expression system for human carbonyl reductase and map the substrate specificities of human carbonyl reductase for anthracyclines.

"Anthracycline Specificity of Recombinant Human Carbonyl Reductase." Principal Investigator, $5000 Period: 7/01/2001-6/30/2002 Funding Agency: Boise State University Faculty Research Grant
The major part of this grant was to develop an expression system for human carbonyl reductase and express, purify, and characterize the kinetics of anthracycline reduction.

Dr. Charlier's Publications, Patents, Abstracts, and Presentations (PDF)