MRCF staff will be attending the annual meeting of the Association of Biomolecular Resource Facilities from March 28 through April 1. During that time the Molecular Core will be staffed part-time by our Career Path Interns, but please be prepared for a delay in sample processing until we return on April 2.
NEW: Next - Generation Sequencing Job Request Form
NOW ACCEPTING PAYMENTS VIA CREDIT CARD
NEW: GALAXY DATA MANAGEMENT SYSTEM
1) Fill out a hard copy of the Job Request Form and drop in our Job Request drop box when placing your samples in the MRCF sample freezer. External users please see instructions below under Shipping. If you need assistance filling out the Job Request Form, follow the steps described in our Submitting Orders document.
New to Submitting Samples?
If you have not previously submitted samples, please download and fill out our mandatory Safety Form and Registration Form, and email completed documents to firstname.lastname@example.org. These forms provide vital information on the types of samples you will be submitting.
2) Email an electronic version of the Job Request Form to email@example.com.
We will process your samples as soon as possible. If this is a rush order, please let us know.
If you have questions about sample preparation and/or shipping, feel free to call us at either (208) 282-4148 or (208) 282-4890.
Please do not use adhesive plate seals on 96-well plates due to the potential for sample cross contamination. Refer to our Submitting Orders information document for recommendations and further information.
To mail samples to the MRCF for sequencing or other analysis, please ship to:
Idaho State University
Molecular Research Core Facility
Gale Life Sciences Building
650 Memorial Drive Mail Stop 8007
Pocatello, ID 83209-8007
- Please include a hard copy of the Job Request Form in the shipped package.
- Please do not use adhesive plate seals on 96-well plates due to the potential for sample cross contamination. Refer to our Submitting Orders information document for recommendations and further information.
Data Viewing and Analysis
Download the free sequence trace viewer and editor from Applied Biosystems. You may also download the freeware version of FinchTV from the Geospiza website. In addition, Sequencher software from Gene Codes Corporation is installed on a Macintosh computer in the MRCF for sequence data analysis, and Gene Mapper software is installed on a computer for Fragment Analysis. If you are an off-campus researcher, download the free Peak Scanner software from Applied Biosystems to view and analyze your Fragment Analysis files.
We provide the following universal primers for DNA sequencing. These can be added to your sample at no additional charge; however, requested addition of these primers needs to be made clear on the Job Request Form.
|Primer||Primer sequence (5'--3')|
|907R||CCGTCAATTCCTTTRAGTTT (R=A or G)|