Price List
DNA Sequencing
- $5.00/sample
- If you submit full (95 samples) or half (47 samples) plates with the template and primer mixed together at the required concentration. This will allow for one pGem control sample per full or half plate. Samples over one half plate in other than groups of 16 will be charged the $7.00/sample rate. Samples must be submitted in 96-well plates or 8-well strip tubes.
- $6.00/sample
- If you submit samples in groups of 16, as long as they are in plates or 8-well strip tubes with the template and primer mixed together at the required concentration. Samples in other than groups of 16 will be charged the $7.00/sample rate.
- $7.00/sample
- If you submit fewer than 16 samples with the template and primer mixed together at the required concentration (0.5 ml tubes, 1.5 ml tubes, 8-well strip tubes, or plates). Due to the 16-capillary format of our sequencer, we may have to wait and batch your samples with those from another researcher.
Fragment Analysis
- $2.00/sample
- If you submit samples in groups of 16, as long as they are in plates or 8-well strip tubes. Includes size standard and processing on the 3130XL. Samples in other than groups of 16 will be charged the $2.50/sample rate.
- $2.50/sample
- If you submit fewer than 16 samples (0.5 ml tubes, 1.5 ml tubes, 8-well strip tubes, or plates). Includes size standard and processing on the 3130XL. Due to the 16-capillary format of our sequencer, we may have to wait and batch your samples with those from another researcher.
DNA sequencing services include sample preparation (cycle sequencing) using Applied Biosystems' Big Dye Terminator chemistry, post cycle sequencing sample purification, and sample processing on our Applied Biosystems 3130XL Genetic Analyzer. After processing we upload data to our Geospiza Gene Sifter data management server, where you can view, download, and print the sequence information and/or original chromatograms. After processing you will receive an e-mail message with instructions for downloading your data from Gene Sifter. A copy of Sequencher DNA sequencing analysis software is available for your use on a Macintosh computer kiosk in the MRCF.
Fragment analysis services include post PCR sample preparation using the appropriate size standard for your samples, and sample processing on our Applied Biosystems 3130XL Genetic Analyzer. After processing we upload your data to our Geospiza Gene Sifter data management server, where you can view, download, and print your data files. After processing you will receive an e-mail message with instructions for downloading your data from Gene Sifter. A copy of GeneMapper software for data analysis is available for your use on a PC computer kiosk in the MRCF.
COST STRUCTURE FOR USAGE OF MRCF SHARED INSTRUMENTATION
Fees provide service contract support and help to cover depreciation costs.
| NanoDrop | $0.15/sample internal ($0.25/sample if you are an external researcher requesting quantification of your samples for sequencing) |
| Bio-Rad VersaDoc | $0.25/minute |
| MJ Thermal Cycler | $10.00/run; During project optimization phase consult with manager about discounted rate. |
| MJ Chromo4 | $20.00/run; During project optimization phase consult with manager about discounted rate. |
| Jouan MR22i | $5.00/hour |
| Sorvall Legend RT | $5.00/hour |
| Nu-Aire Clean Bench | $2.00/hour |
| Hemco Fume Hood | $2.00/hour |
| Nanopure water | $0.25/liter |
| Bio-Rad VersaDoc prints | $0.10/print |
| HP LaserJet prints | $0.10/print |
| Tri-Carb Liquid Scintillation Counter | $0.25/sample for first 20 samples, then $0.10/sample |