MRCF staff will be attending the annual meeting of the Association of Biomolecular Resource Facilities from March 28 through April 1. During that time the Molecular Core will be staffed part-time by our Career Path Interns, but please be prepared for a delay in sample processing until we return on April 2.
Forms & Resources
Imaging Request Form
Please fill out this form pertaining to the type of cells/tissue and your imaging needs prior to using the confocal laser scanning microscope.
Please email Lisa McDougall (email@example.com) with a time that is open and works best for you. We will try to work with everyone's schedules so that the microscope is used in the most efficient way.
Protocols and User Guides
General IHC Protocol
This is a general protocol for immunolabeling of cells from culture or tissue cryosections. The protocol also includes a description of the Alexa Fluor secondary antibodies that will work with the AICF's confocal laser scanning microscope.
Guide to Alexa Fluors
This document lists the available Invitrogen Alexa Fluors - secondary antibodies that are conjugated to a specific fluorphore. The document shows the excitation and emission spectra for each Alexa Fluor.
Confocal Laser Scanning Microscope Quick Guide
Multiphoton Microscope Quick Guide