Leica DMRB
This manual microscope facilitates phase contrast, DIC, darkfield, and epifluorescence imaging. The system is outfitted with an EL6000 external light source, SPOT CCD camera and software for digital color image acquisition and processing.
Available Filters:
Figure 1. DMRB brightfield image of mammalian cartilage (top) and mammalian compact bone (bottom). Both tissues were stained with a general tissue stain.
| Set ID | Filter Set Type | Exciter | Emitter | Mirror |
|---|---|---|---|---|
| XF14 | GFP | 420/20 | 540/40 | 480lp |
| 49002 | AF 488, GFP | 470/40 | 525/50 | 495lp |
| XF34 | Rhodamine, PI, EB, AF 546/555 | 540/20 | 675/85 | 560lp |
| 49004 | Ds Red | 545/25 | 605/70 | 565lp |
| 49008 | Texas Red | 560/40 | 630/75 | 585lp |
| 49006 | Cy 5 | 620/60 | 700/75 | 660lpxr |
| 11000v3 | DAPI | 350/50 | 420lp | 400lp |
| 31001 | FITC, GFP, Fluo3, Acridine Orange | 480/30 | 535/40 | 505lp |
Available Objectives:
| Ring color | Platform | Magnification | Immersion | ID Number |
|---|---|---|---|---|
| Brown | N Plan | 2.5X NA 0.07 | Air | 50683 |
| Red | HC PL Fluotar | 5X NA 0.5 | Air | 506504 |
| Yellow | PL Fluotar | 10X NA 0.30 | Air | 506010 |
| Green | PL Fluotar | 20X NA 0.50 | Air | 506003 |
| Blue | PL Fluotar | 40X NA 0.70 | Air | 506014 |
| White | FL Fluotar | 100X NA 1.3 | Oil | 506008 |