Leica DMRA
This fluorescence microscope provides all the basic features of the DMRB with the added capabilities of automated z-stack acquisition and filter wheel control. The system is outfitted with an EL6000 external light source, Hamamatsu DCAM monochrome camera and IPLab software for digital image acquisition and analysis.
Figure 1. Triple fluorescence labeled bovine pulmonary artery. Image was captured on the DRMA microscope. Mitochondria (red), Actin (green) and nucleus (blue).
Available Filters:
| Set ID | Filter Set Type | Exciter | Emitter | Beam Splitter |
|---|---|---|---|---|
| 513848 (A4) | DAPI, Hoechst 33258/33342, Alexa 350 | 360/40 | 470/40 | 400lp |
| 71000A | Fura 2 | 340/10 | 510/80 | 400lp |
| 513852 | GFP | 470/40 | 525/50 | 500lp |
| 513849 (L5) | FITC/GFP/Cy2 | 480/40 | 527/30 | 505lp |
| 513850 (N3) | Alexa 546 | 546/12 | 600/40 | 565lp |
Available Objectives:
| Ring color | Platform | Magnification | Immersion | ID Number |
|---|---|---|---|---|
| Yellow | HC PL APO | 10X NA 0.40 | Air | 506511 |
| Green | HC PL APO | 20X NA 0.70 | Air | 506513 |
| Blue | HCX PL APO | 40X NA 0.85 | Air | 506140 |
| Navy Blue | HCX PL APO | 63X NA 1.20 | Water | 506131 |
| White | HCX PL APO | 100X NA 1.4X | Oil | 506038 |