Molecular Research Core Facility

Confocal Laser Scanning Microscope


The new Olympus FluoView™ FV1000 is the latest in point-scanning, point-detection, confocal laser scanning microscopes designed for today's intensive and demanding biological research investigations. Excellent resolution, bright and crisp optics, and high efficiency of excitation, coupled to an intuitive user interface and affordability are key characteristics of this state-of-the-art optical microscopy system. Our system has four simultaneous detection channels, including one for DIC and two spectral channels. Source.


Need help deciding which fluorophore to use? Click here to open a detailed Invitrogen Alexa Fluor guide.

Available Laser Lines:

Lasers Lines
405 nm
458 nm
488 nm
515 nm
559 nm
635 nm

Available Objectives:

Magnification Platform Immersion
10X NA 0.40 UPLanSAPO Air
20X NA 0.75 UPLanSAPO Air
40X NA 0.90 UPLanSAPO Air
40X NA 1.30 UPLanFLN Oil
60X NA 1.42 PLanAPO Oil
100X NA 1.40 UPlanSAPO Oil

Figure 1: Confocal miscroscope images of autofluorescence in pond algae found in Pocatello, Idaho (left) and Ascomycotes sp., cup fungus (right). Two different emission wavelengths are detected in each sample.


Figure 2: Paramecium, A) Projected z-stack of fluorescence images of cilia structures of multiple microorganisms. B) Differential interference contrast (DIC) image of the same group of paramecium. Both images were captured simultaneously on the confocal laser scanning microscope.


Figure 3: Autofluorescence in Pinus laricio. The confocal laser scanning microscope was used to acquire a lambda scan, which samples a user-defined range of wavelengths of fluorescence emission. Lambda scans are useful for identifying peak fluorescent wavelengths when using uncertain fluorophores and for identifying autofluorescence in samples.


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